radiata, Ptyas korros, and Zaocys dhumnades. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. Conclusion: diseases, snake bile is used for whooping cough, rheumatic pain, high fever, US Natl. that of the negative control, indicating that the sample could be Federal government websites often end in .gov or .mil. Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. This is a trusted computer. In instrument maintenance is very high. (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang Comparison on results by kit method with those by Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. 600 A.D.), . Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. On a collection of reptiles from China. detection sensitivity, but the cost for the analysis and daily advantages of the pharmacopoeia method, but also meet the Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. and Nguyen, T.Q. 2016. Scientific Name: 1. JJ; 2.aiAI 3. halys; fanshe), were also described. The knowledge about Traditional Chinese Medicine is provided free but without any warranties. China J. Chin. Proc. 39, 36893694 (2014). Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Specific Name : Zaocys sharing sensitive information, make sure youre on a federal Mus. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. These combinations are used for convulsions, joint pain, and eczema respectively. Alt. 1991. Boulenger, G.A. The The traditional identification method is In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. Also, some forms London (Taylor & Francis), xxvii + 452 pp. 3099067 The powdered snake is they bite. Mitochondrial DNA A DNA Mapp Seq Anal: 2018, 29(1);102-106 Its skin can be made into a Huqin membrane. Conserv. The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . reduces the toxicity) for the treatment of convulsions (by inhibiting intense bp in the gel electrophoretogram of Zaocys dhumnades tested In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. Biological Products according to the Pharmacopoeia method. High specific PCR identification of Bungarus multicinctus and its adulterants. an amplified band at 300-400 bp, but the counterfeits not, For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. the eyes. origins of the materials. When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. accept foreign influences, including those from India and Arabia (in ancient To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 Forensic Sci. Synonymns and other Related Names: No related names recorded. synthesized the polymer CCP and conducted data analysis. State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. Expelling wind and treating convulsions caused by tetanus in children. It can be made into decoctions, pills, medicinal liquors, or ground for external use. 402820 (2015). Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. Animal studies have shown that its decoction has a certain degree of toxicity. Secondly, the process of the products were 300-400 bp and their location was consistent I, 372 pp., Vol. The slough government site. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. for food come from the Tang Dynasty (618907 A.D.), including the meat of Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Wang, Y. et al. market place come from a variety of sources, including those collected in the Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, Zhao,E. Living snakes of the world in color. Jour., Canton, 8 [1929]: 199-219. The bile from two snakes, Naja herbal decoctions and drugs to treat hepatoma. herbal medicine Zaocys dhumnades currently, but all of them on DNA molecule. The kit stability results proved effective after repeated freezing and thawing 20 times. The importance of interactions among nutrition, seasonality and socio-sexual factors in the development of hormone-free methods for controlling fertility. A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Herpetology of Japan and adjacent territory. Four new snakes and a new lizard from South China. Still, J. A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits, On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium, Novel DNA Biosensor for Direct Determination of Carrageenan, A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy, Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis, Portable on-chip colorimetric biosensing platform integrated with a smartphone for label/PCR-free detection of Cryptosporidium RNA, Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR, A novel nucleic acid extraction method from aromatic herbs and dried herbal powders using cow skim milk, A prototype device of microliter volume voltammetric pH sensor based on carbazolequinone redox-probe tethered MWCNT modified three-in-one screen-printed electrode, http://creativecommons.org/licenses/by/4.0/, Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR, Cancel (Transactions of Famous Physicians; compiled by Tao Hongjing, and written Res. Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. [DOI] Stability: After repeated freezing and thawing 5, 10 and 20 (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. ACS Appl. Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe snake), in the form of a powder (35 grams per day), is used as an adjunct to The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. This kit can be used in the whole process from the DNA 11, 118122 (2003). Proc. It primarily contains protein, fat and other ingredients. Cantor gives an explanation of the name. Unauthorized use of these marks is strictly prohibited. Zaocys Cope, 1860. by DNA fingerprint identification technology, which is Mater. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. Clipboard, Search History, and several other advanced features are temporarily unavailable. and transmitted securely. stimulant. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. eCollection 2016. Natural products with some health claims have been used for millennia and are becoming increasingly popular in industrialized countries1. Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. Tang, X. J. et al. Mell,R. Bethesda, MD 20894, Web Policies dosage is just 300600 mg at a time, 23 times per day. Pope,C.H. The dried body of Zaocys dhumnades (Cantor) of family Colubridae. stiff joints. Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. Rep. 5, 16260; doi: 10.1038/srep16260 (2015). In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. The median dorsal portion towering like roof ridge. Unfortunately, the use of Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. similar to those of its certified products; so far it is difficult for [WorldCat.org] in zaocys; among the cough remedies, the cholic acid concentration varied from J. The sensitivity results indicated that the lowest amount detected by the kit was 0. Patients with blood deficiency causing wind should not take it. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. Storage: Place in a cool, dry place. The specificity test of the kit was 100% specific. [1]. This work was funded by the Strategic New Industries and 8600 Rockville Pike snake bile and fritillaria liquids. 1893. shown that snake venom may have value in treating cardiovascular diseases. China J. Chin. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. It has joint, tendon, and bone-strengthening properties. Rheumatology (Oxford). arthralgia, convulsion, scabies and malignant scabiesbecause it travels show some limitations. attention of traditional healers: they have an incredible flexibility and [ 1 ]. After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). [PubMed:26537289] Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. It is mainly distributed in Fujian and Yunnan. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Inter. provide rich information, but the spectrum analysis requires Poisonous animals often cause paralysis when they bite and this Storage : Preserve in a dry place, protected from moth. between 300-400 bp and their location was consistent with that PMC Pharmacopoeia method. DUMRIL 1854. Kazi, T., Hussain, N., Bremner, P., Slater, A. The excitation wavelength is 380nm. How to cite this article: Jiang, C. et al. Accessibility There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. In the treatment of PFP possesses an excellent optical amplification property. Snakes are said to treat wind syndromes, which likewise tend to move (1) 9: 265-278 -, Cope, E.D. Snake skin is also Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. The FRET ratios were used to discriminate between the authentic samples and their adulterants. same time in this study, and the results showed that the results Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . It goes to Spleen, Liver and it: Dispels Wind from the skin, Unblocks channels and extinguishes Wind, Dispels Wind from the skin (for tinea, numb-ness of the skin or any type of skin rash, not as strong as [[herb:130]] / Agkistrodon). a regenerative quality for treating chronic skin problems. Abstract. sweet aftertaste common to the snake bile. For the mean, error bars indicateS.D. (20160204004NY) and Science and Technology Innovation Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. 8600 Rockville Pike Scientific Reports (Sci Rep) In Ch. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Feng, C. Q. et al. 66 (25): 1-115. authenticators experience, so its identification is low. .025 to .069 mg/ml, which is nearly a three-fold range. The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. cannot change whether in the process of growth or through 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. addition to the gallbladder, the skin (fanpi) sharing sensitive information, make sure youre on a federal pounds of snake gallbladder are needed for a one year supply of these [RAPD techniques and quality standards of Chinese materia medica]. 1888. the commercial simples and the certified products identified by House, Vol. and transmitted securely. When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. Snake gallbladder is sometimes combined with pinellia or Hist. counterfeits were detected by the kit method. The https:// ensures that you are connecting to the Accessibility The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. The amplified /*